Chapter 9

NCERT
Class 11
Biology
Solutions
Find out a qualitative test for proteins, fats and oils, amino acids and test any fruit juice, saliva, sweat and urine for them?

Question:

 Find out a qualitative test for proteins, fats and oils, amino acids and test any fruit juice, saliva, sweat and urine for them?

Answer:

We can identify biomolecules using a few simple lab tests:

  • Proteins - Biuret Test: Add Biuret reagent; a violet colour confirms protein.
  • Fats & Oils - Sudan III Test: Sudan III gives a red/orange layer if lipids are present.
  • Amino Acids - Ninhydrin Test: A purple colour shows the presence of amino acids.

Testing common samples

  • Fruit juice: Usually shows a positive ninhydrin test because it contains some amino acids.
  • Saliva: Gives a positive protein test since it has the enzyme amylase.
  • Sweat: Mostly water and salts, so tests are usually negative or very weak for proteins.
  • Urine: Normally negative for proteins, fats, and amino acids (their presence usually indicates a health issue).

These quick qualitative tests help identify what biomolecules are present in everyday samples.